Flow-injection Post-chemiluminescence Determination of Furazolidone in Animal Feeds

The post-chemiluminescence (PCL) reaction was observed with the mixture solution after completion CL reaction of luminol-H2O2 in present of Furazolidone (FZD). A novel method for the determination of FZD was established by flow-injection PCL. The CL intensity responded linearly to the concentration of FZD in the range 1.0×10 −7 -1.0×10 −5 g mL -1 , with a linear correlation of 0.9949. The detection limit for FZD was 1.96×10 −8 g mL -1 (S/N=3). The relative standard deviation (RSD) was 3.5% (n=11, c=1.0×10 −6 g mL -1 ). The proposed method has been applied to the determination of FZD in animal feeds and the results were consistent with those obtained by the HPLC-UV method. A possible mechanism for the PCL reaction was also discussed by the investigation of CL kinetic characteristics, CL spectra and UV-Vis absorption spectra.